바로가기메뉴

본문 바로가기 주메뉴 바로가기

logo

  • P-ISSN2765-2203
  • E-ISSN2765-2211

Development of a Plastid DNA-Based Maker for the Identification of Five Medicago Plants in South Korea

Proceedings of the National Institute of Ecology of the Republic of Korea, (P)2765-2203; (E)2765-2211
2022, v.3 no.4, pp.212-220
https://doi.org/10.22920/PNIE.2022.3.4.212
Il Ryong Kim (LMO Team, National Institute of Ecology)
A-Mi Yoon (LMO Team, National Institute of Ecology)
Hye Song Lim (LMO Team, National Institute of Ecology)
Sunghyeon Lee (LMO Team, National Institute of Ecology)
Jung Ro Lee (LMO Team, National Institute of Ecology)
Wonkyun Choi (LMO Team, National Institute of Ecology)
  • Downloaded
  • Viewed

Abstract

DNA markers have been studied and used intensively to identify plant species based on molecular approaches. The genus Medicago belongs to the family Fabaceae and contains 87 species distributed from the Mediterranean to central Asia. Five species of Medicago are known to be distributed in South Korea; however, their morphological characteristics alone cannot distinguish the species. In this study, we analyzed the phylogenetic relationships using collected five species of Medicago from South Korea and 44 taxa nucleotide information from NCBI. The constructed phylogenetic tree using gibberellin 3-oxidase 1 and tRNALys (UUU) to maturase K gene sequences showed the monophyly of the genus Medicago, with five species each forming a single clade. These results suggest that there are five species of Medicago distributed in South Korea. In addition, we designed polymerase chain reaction primers for species-specific detection of Medicago by comparing the plastid sequences. The accuracy of the designed primer pairs was confirmed for each Medicago species. The findings of this study provide efficient and novel species identification methods for Medicago, which will assist in the identification of wild plants for the management of alien species and living modified organisms.

keywords
DNA markers Medicago Phylogenetic tree Plastid genome Species specificity
Submission Date
2022-08-16
Revised Date
2022-09-14
Accepted Date
2022-09-15

Proceedings of the National Institute of Ecology of the Republic of Korea