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  • P-ISSN2765-2203
  • E-ISSN2765-2211

A Multiplex PCR Method for the Detection of Genetically Modified Alfalfa (Medicago sativa L.) and Analysis of Feral Alfalfa in South Korea

A Multiplex PCR Method for the Detection of Genetically Modified Alfalfa (Medicago sativa L.) and Analysis of Feral Alfalfa in South Korea

국립생태원보, (P)2765-2203; (E)2765-2211
2020, v.1 no.1, pp.83-89
https://doi.org/10.22920/PNIE.2020.1.1.83
ChoiWonkyun(Wonkyun Choi) (Division of Ecological Safety, National Institute of Ecology)
KimIl Ryong(Il Ryong Kim) (Division of Ecological Safety National Institute of Ecology)
LimHye Song(Hye Song Lim) (Division of Ecological Safety, National Institute of Ecology)
LeeJung Ro(Jung Ro Lee) (Division of Ecological Safety, National Institute of Ecology)
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초록

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

keywords
Alfalfa Genetically modified organism Genetically modified organism detection Medicago sativa Multiplex polymerase chain reaction

Abstract

Methods for detecting the presence of genetically modified (GM) crops are evolving to comply with legislation and to enhance monitoring by biotechnology companies and regulators. In order to cover a broad range of detection methods for a new GM crop, conventional multiplex PCR methods are required. Based on the genetic information on three GM alfalfa varieties (J101, J163, and KK179), which were recently approved in South Korea, we developed a fast, reliable, and highly specific multiplex polymerase chain reaction (PCR) method with basic PCR equipment and inexpensive reagents. To validate and verify the newly developed multiplex PCR method, we applied a limit of detection assay and random reference material analysis. We also monitored the unintentional environmental release of GM alfalfa in South Korea by performing the multiplex PCR analysis with 91 feral alfalfa specimens collected from 2000 to 2018. Our methodology is a sensitive, simple, quick, and inexpensive tool for detecting and identifying three GM alfalfa varieties.

keywords
Alfalfa Genetically modified organism Genetically modified organism detection Medicago sativa Multiplex polymerase chain reaction
투고일Submission Date
2020-09-18
수정일Revised Date
2020-10-06
게재확정일Accepted Date
2020-10-06

국립생태원보